Biopharmaceuticals (Biological Source)
Biopharmaceutical manufacturing processes have high costs due to the multistep purification process, which is required to separate the many components generated during the cell culture step. The manufacturing process is roughly divided into upstream processes, including cell culture, and downstream processes, including purification. Purification using chromatography in the downstream process includes three steps: initial purification (capture), intermediate purification and final purification (polishing). In each step, the packing material (bulk medium) and chromatography device are chosen depending on the intended purpose.
Based on the interaction between the solid stationary phase and biomolecules, chromatographic techniques can be summarized into five classes: (i) affinity, (ii) ion-exchange, (iii) hydrophobic interactions, (iv) size exclusion and (v) mixed-mode chromatography.
Zeochem’s irregular shaped ZEOprep silica is a column packing material approved over decades for allowing highly reproducible results. Used primarily in upstream and downstream stages of biopharmaceutical processes, ZEOprep and ZEObead products offer high performance, high permeability and excellent selectivity, in addition to effective and reproducible separation.
Zeochem’s ZEOsphere DRP Mixed-Mode silica is an excellent choice for the purification of charged intermediates and as a polishing step. ZEOsphere DRP Mixed-Mode can substantially increase recovery/yield while decreasing organic solvent usage due to improved selectivity capabilities.