Major analytical techniques used in research & development, quality control and quality assurance are HPLC chromatography and NMR spectroscopy. Zeochem offers silica gel packing materials for various types of chromatography and deuterated substances for the NMR spectroscopy.

Liquid Chromatography is the most important tool in any chemical analytic. Silica gel is considered the best-performing stationary phase in chromatographic separations. The various types of chromatographic techniques in use require different types of silica gels. Our wide range of silica gels meets these requirements: ZEOprep irregular shaped silica, ZEObeads spheroidal silica and ZEOsphere spherical silica. These forms are available as both bonded and unbonded silica.

Zeochem ZEOprep irregular silica and the spheroidal ZEObeads products are very reliable packing materials for analytical laboratory kits, SPEs and QuEChERS.

Zeochem’s ZEOsphere materials analytical HPLC phases/columns are used in early development and discovery stages of biopharmaceutical, nutraceutical and food companies, as well as in medical and research laboratories. Analytical stationary phases for ZEOsphere silica are superb in performance. Separation methods can be developed and directly transferred to the preparatory phase via easy scale-up principles.

For analytical separations, we offer ZEOsphere Classical silica (e.g., C18, C8, C4, etc.) for any reversed phase application and ZEOsphere DRP Mixed-Mode stationary phases for charged molecules like peptides, insulins (analogues) and oligonucleotides. These products are available in analytical, semi-preparative columns or in small bulk quantities.

Standard ZEOsphere stationary phases are available in pore sizes 100Å, 120Å, 200Å, 300Å and 1000Å and in analytical particle sizes 3µm for standard HPLC or UHPLC applications (only for ZEOsphere DRP) or 5µm (for ZEOsphere Classic).

Scaling up is easier when using an HPLC media that provides nearly identical performance across all particle sizes and with increases in column diameter. Any mobile phase conditions developed on a ZEOsphere analytical column can be easily transferred to a 10 μm or 15 μm preparative column with an equivalent resolution, selectivity and proportional mass loading.


Classical ZEOsphere silica and ZEOsphere DRP Mixed-Mode analytical HPLC phases/columns provide excellent performance and reliable and robust solutions for your quality control activities.

Quality control is an essential operation of the biopharmaceutical, nutraceutical and other industries. For example, biopharmaceutical drugs must be marketed as safe and therapeutically active formulations with consistent and predictable performance.

New pharmaceutical grade compounds are being synthesized at an accelerated rate, and in response, more advanced and robust analytical methods are being developed for the evaluation of new drug substances and drug products. HPLC and LC-MS are playing an important and critical role as a quality control analytical method for qualitative and quantitative analysis.

The importance of HPLC or LC-MS used in biopharmaceutical industries is falling under the stringent regulations established by the U.S. Food and Drug Administration (FDA), European Medicines Agency (EMA) and other regulatory agencies. This type of regulation requires biopharmaceutical companies to test the quality of their products using either HPLC or LC-MS before they can be sold to the global market.

Our innovations in phase technology led to a selectivity revolution. ZEOsphere DRP Mixed-Mode in attraction-repulsion mode offers enhanced selectivities, making your quality control less stressful.

ZEOsphere DRP Mixed-Mode materials can contribute to achieving higher throughput, lower costs and faster time to market for routine analysis of NCEs or generic drugs.

Our ZEOsphere analytical HPLC phases/analytical columns are excellent choices for identification and quantification applications in your quality control or scientific laboratory.

Zeochem's standard ZEOsphere stationary phases are available in pore sizes 100Å, 120Å, 200Å, 300Å and 1000Å and in analytical particle sizes 3µm for standard HPLC or UHPLC applications (only for ZEOsphere DRP) or 5µm (for ZEOsphere Classic).

For standard identification/quantification application, we offer ZEOsphere Classical C18, C8, C4, Si, CN, NH2, DIOL, Phenyl, Phenyl-Hexyl and Phenyl-Butyl phases. For charged molecules like peptides, insulins (analogues) and oligonucleotides, we recommend our ZEOsphere DRP Mixed-Mode stationary phases. ZEOsphere DRP Mixed-Mode phases are available as RP/SAX and RP/SCX.

Nuclear magnetic resonance spectroscopy, most commonly known as NMR spectroscopy, involves the technique of observing magnetic fields around the nuclei. Deuterium Labeled Compounds are used as solvents in NMR spectroscopy. Zeochem's standard product range covers the daily requirements of NMR end users. Our products are pre-packed in custom-designed packaging and immediately dispatched when minimum order quantities are met.

Custom Solutions

Zeochem's technical service team, engineers and chemists have decades of experience and are here to answer questions, troubleshoot and find solutions to your most challenging problems.

Related Products

Classical Reversed Phase – C18

ZEOsphere C18 phases are the most preferred as they offer an excellent range of hydrophobic separation power along with high surface area coverage.

Classical Reversed Phase – C4

ZEOsphere C4 is commonly used to separate large macromolecules such as proteins

Classical Reversed Phase – C8

An excellent range of hydrophobic separation power along with high surface area coverage, but when less retention compared to a C18 is needed.

Deuterium Used as Tracer or Marker

Zeochem offers deuterium oxide in different isotopic qualities, which are used in the tracer and marker fields for food, beverage, agriculture and hydrology industries.

NMR Solvents

Zeochem offers a broad range of NMR solvents and deuterium labeled products for routine analytics and research & development. The main application is for use in NMR spectroscopy.

ZEObeads 50 C18 / 20-30µm

Spheroidal shape alkyl-bonded silica used in reversed-phase chromatography, preferably where superior flow performance and selectivity are needed.

ZEObeads 50 C8 / 20-30µm

ZEObeads 50 C8 / 20-30um is a spheroidal shaped silica with a reduced alkyl chain length, slightly less hydrophobic in reverse phase chromatography. The shorter alkyl chain length allows in some cases a better interaction with the target compound.

ZEObeads 50 Diol / 20–30µm

ZEObeads 50 NH2 / 20-30um is a spheroidal shaped amine bonded silica. It is used in normal phase chromatography for target compounds with more alkaline pH and as an excellent metal scavenger under conditions of superior flow performance.

ZEObeads 50 NH2 / 20-30µm

ZEObeads 50 NH2 / 20-30um is a spheroidal shaped amine bonded silica. It is used in normal phase chromatography for target compounds with more alkaline pH and as an excellent metal scavenger under conditions of superior flow performance.

ZEOprep 60 C18 / 40-63µm

Alkyl-bonded silica for reversed-phase chromatography is a robust and reliable packing material, widely used in industrial separations as well as...

ZEOprep 60 C8 / 40–63µm

Alkyl-bonded silica with shorter chain length and slightly reduced hydrophobicity which is used in reversed-phase chromatography. The shorter alkyl length...

ZEOprep 60 CN / 40-63µm

Cyan-bonded silica is more polar than C18/8 bonding but is mostly used in reversed-phase chromatography for polar compounds and for...

ZEOprep 60 Diol / 40-63µm

Diol-bonded silica, more polar than C18/C8 types. Used in reversed- as well as in normal-phase chromatography, it is the product...

ZEOprep 60 NH2 / 40-63µm

Amine-bonded silica is mostly used in normal-phase chromatography and serves as an efficient metal scavenger in batch applications, best for...

ZEOprep 60 NH2P / 40–63µm

Primary, secondary amine-bonded silica and is more polar than C18/C8 phases. It is used in reversed-phase as well in normal-phase...

ZEOprep 60 SAX / 40-63µm

Silica-bonded for anion exchange chromatography.

ZEOprep 60 SH / 40-63µm

Thiol-bonded silica most efficiently serves as a metal scavenger, but it is also used as a means to remove palladium...

ZEOprep 90 C18 / 40-63µm

Alkyl-bonded silica with 90 angstrom pore size that has proved to be a robust packing material in particular for flash...

ZEOprep 90 C18 / 60-200µm

Alkyl-bonded silica with 90 angstrom pore size and a larger particle size distribution which provides a higher flow rate under...

ZEOprep 90 C8 / 40-63µm

Alkyl-bonded silica with 90 angstrom pore size with shorter alkyl chain length and slightly reduced hydrophobicity. It is used in...

ZEOprep 90 CN / 40–63µm

Cyan-bonded silica with 90 angstrom pore size, more polar than C18/8 bonding but mostly used in reversed-phase chromatography for polar...

ZEOprep 90 NH2 / 40-63µm

Amine-bonded silica with 90 angstrom pore size, mostly used in normal phase chromatography. It serves as an efficient metal scavenger...

ZEOprep Bonded Silica

Bonded ZEOprep silica is used in liquid chromatography, flash chromatography and solid phase extraction. It can be used as scavengers...

ZEOprep Unbonded Silica

Unbonded ZEOprep silica is mostly used as column packing material for preparative chromatography and the purification of natural products, food...

Frequently Asked Questions

Heavy water is a valuable material and should not be disposed. You can return your depleted heavy water to Zeochem. Please contact us at for more information.

  • The larger the molecular weight of the target molecule to be separated the larger the pore size needs to be. The sample molecules need to be able to interact with the pores of the particle to create an efficient separation mechanism. If the pores are smaller than your target molecule it will not be retained and elute immediately.
  • The larger the average pore size of the material the smaller will be the effective surface area. If you have less surface area available, your separation is generally less efficient. It is important to choose the right pore size for your target molecule.
  • Size and distribution of the silica particles effect the packing efficiency of the columns and therefore process performance.
  • The higher the packing density, the higher the surface area, the more interaction between eluent and separation material resulting in higher efficiency separation. However, the higher the packing density, the smaller the mean particle size is and therefore the higher the backpressure will be.

Bonding different chemical groups onto the surface of bare silica results in functionalized silicas which give higherselectivity towards specific molecules.

  • Bare/Silica has a high polar surface, it is the most popular stationary phase, ideal for conventional applications (normal phase separations, non-polar target molecules, pharmaceuticals, natural products)
  • C4; suited to molecules with large hydrophobic regions, peptides, proteins. Usually combined with a large pore size for big biomolecules (>1000Da)
  • PHE/phenyl; moderately nonpolar for aromatic compounds, aflatoxins, caffeine, phenols.
  • C8; highly hydrophobic pesticides, peptides, drugs
  • C18; more apolar than C8 and in reversed phase chromatography higher retention than C8. Has a non-polar surface, ideal for pharmaceuticals, steroids, fatty acids, peptides, proteins, pesticides, PCB’s
  • Amino/NH2has a medium polar surface, ideal for carbohydrates and nitrogen containing heterocycles and amines. Scavenger applications.
  • Diolhas a lesspolar surface (than bare silica) and still many hydroxy-groups, ideal for lipids; Our best option for many SFC applications.
  • Cyano/CN unique selectivity for polar analytes with widely different chemistry eg. Cyclosporine, carbohydrates
  • Thiol/SH; Mainly used for scavenging
  • SCX/SO4:Ion Exchange for strong cation
  • SAX/TMA; Ion exchange for weak anion
  • NH2P; Ion exchange for scavenging
  • Morphology of the silica particles; meaning bead shape
  • Surface area: this is a function of particle- and pore size
  • Surface functionalisation
  • Particle size distribution in terms of range and actual distribution of particles within the range (D10/D50/D90)
  • Irregular silica beads to be used in low -medium pressure systems
  • Spheroidal Silica beads can be used in low to high pressure systems
  • Spherical silica beads primarily used for high-resolution high-pressure systems

Zeochemproduces deuterium-labelled compounds. The enrichment is shown in percentage D (Deuterium). Chemical purity refers to the content of a particular substances. For NMR solvents, it is usually tested by GC in area percentage.

Where does water show up in NMR?

We recommend the use of glass ampoules. Each ampoule is sealed and inspected visually. We have ampoules in boxes of 10 with 0.5mL, 0.6mL, 0.75mL each available.

DMSO-d6, Chloroform-D and Methanol-d4 are the most used solvents for NMR applications due to its wide range of solvent constraints as well as its simple spectrum and high boiling point.